Evaluation of hepatoprotective activity of standardized Shodhita Croton tiglium Linn. Seed
AUTHORS : Amit Kumar Yadav, Ashish Mishra, Santosh Kumar Maurya DOI No. : NA DOI Link. : NA Background: Liver disorders are a serious problem which lacks an effective medicine from modern pharmaceutical. Plant based system of medicine, Ayurveda, which is practiced since many centuries in South East Asian countries offers a number of treatment approaches for liver diseases such as Aushadhi (medicines), raktamokshan (blood leaching), virechan (therapeutic purgation) and upavasa (fasting). However, among them virechan (therapeutic purgation) is mention as best. One of the strong purgative drug Croton tiglium Linn. (Euphorbiaceae) was undertaken after Shodhan (detoxification) in the study to evaluate its effect on liver biomarkers using CCl4 as hepatotoxin. Shodhan with cow milk reduces the amount of phorbol esters and remove the crotonic acid from the seeds of C. tiglium. Both the compounds are responsible for its purgative property.
Method: Standardized crude extract of Shodhita seeds of C. tiglium (CTS) were obtained from Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur (U.P) India. Thirty six adult rats were divided into six groups (n=6). Group 1 serve as control group, group 2 as hepatotoxin treated group, Group 3 as standard group and group 4-6 serves as test group. CCl4 (1.0 mL/kg b. w. 50% v/v with olive oil; p.o.) was used as hepatotoxic while silymarin (100mg/kg b.w.; p.o.) as reference drug. Silymarin and CTS (20 and 30 mg/kg b.w. ; p.o) was given to their respective grouped animal for seven days. The animals were challenge by CCl4 only on sixth and seventh day. Biochemical parameters (total bilirubin, direct bilirubin, SGPT, SGOT and ALP) were estimated in blood while antioxidant activity (LPO and SOD) was performed with liver tissues. The effect was further confirmed by histopathological changes of liver tissues. Result: The animals treated with CTS showed significant (p< 0.05) improvement in the level of serum enzymes, total bilirubin and direct bilirubin which are increases after administration. The drug also attenuates the CCl4 induced oxidative stress. Furthermore, the histological examination also supports the biochemical reports.
Conclusion: The finding of the liver biochemical markers and histopathological changes in the present study reveals that the CTS might be able to protect liver from acute damages caused by CCl4.